All liquid assay measurements were done in duplicate and repeated at least three times on different days (n ≥ 6); cell assay measurements were done in triplicate and repeated at least three times as well (n ≥ 9).
Transfections were done in duplicate and repeated at least 3 times in independent experiments.
Experiments were done in duplicate and repeated 3 4 times on separate occasions.
Transfection experiments were done in duplicate and repeated at least three times.
Typically, the reactions were performed with duplex DNA, purified HP0593 MTase, and [3H] AdoMet at 25°C for 10 min. Each experiment was done in duplicates, repeated three times, and the average values reported.
Each experiment was done in triplicate repeated three separate times.
The approach does not assume that all variances or all covariances will be equal (as is done in classical repeated-measures ANOVA or in a mixed model using only a random subject effect).
"We don't believe in repeating what teachers are doing during the day," said Lenore Neier of the Y.M.C.A. of Greater New York, which runs school-based programs for 11,000 children, featuring plays and journal writing, among other activities.
The first determination of the absolute gravity at the reference site GPK1 of the Soultz network was done in April 2013 and repeated in October 2013.
All experiments were done in triplicates and repeated three times.
All experiments were done in duplicates and repeated three times.
