After the controller was designed and implemented a series of experiments were done in varied conditions of mass and signal input.
But bad ideas do thrive in conditions of maximum claptrap.
Introduced species usually exhibit changes in genetic variation, population structure and phenotypic characteristics due to selective pressures that do not occur in conditions of demographic equilibrium, which may reflect previous evolutionary histories, stochastic events and selection (Keller and Taylor 2008).
A 24 cycle-PCR was done in standard conditions with 10 μmol of fluorescent reverse primer and 20 μmol of the direct one, in a final volume of 50 μL.
A multiple 26-cycle PCR was done in standard conditions using 10 μmol of direct and reverse primers and 40 μmol of the allele-specific primer, in a final volume of 50 μL.
According to previous work done in similar conditions [ 25], a sample size of 20 dogs ensured a difference of 4.2%BW in the PVF consistent with an effect size of 0.9 could be detected assuming 80% power, a SD of 4.5 and a 5% significance threshold.
Measurements were done in outside conditions next to the entrance of the chair lift and were performed to the exact point in time.
Penile echo-color-Doppler was done in basal conditions and after intracavernous injection of 10 μg prostaglandin E1 (PgE1), and the peak systolic velocity (PSV) and end-diastolic velocity (EDV) were recorded 5, 10, 15, 20, and 25 min after the injection in the proximal portion of the penis.
The experiments on mice have been done in the appropriate conditions of husbandry, experimentation and care, because our animal house is controlled by an ethic comity.
This raises the possibility that CHOP may be required for maintaining a high basal expression of Bim through a mechanism that does not affect Bim in conditions of increased ER stress.
