For each of the tested conditions, 35 μL of DON stock solution (100.8 μg mL−1) were evaporated to dryness under a gentle nitrogen stream in triplicate and the residue was re-dissolved in 7 mL of the hydrolysis agent.
Flowering ears were either treated with the DON inoculation solution or with water (mock) according to the following procedure: at time point zero, 10 μL DON were injected in each of two adjacent spikelets in the lower part of a flowering ear.
Sensorgrams were recorded during the injection of a mixture of 40 μL of the monoclonal antibodies (aDON 20 μg/mL and aZEN 5 μg/mL) and 40 μL of the multianalyte standard solution containing DON and ZEN toxins (0, 0.1, 1, 2.5, 5, 10, 25, 50, 100, 1,000 ng/mL).
Two sets of standard stock solutions in acetonitrile were prepared for spiking experiments: (i) individual solutions of DON, D3G, 3ADON, and 15ADON at a concentration of 10 μg mL−1 for the HM validation, and (ii) a mixed solution of all toxins used at level of 10 μg mL−1 for the NHM validation.
Standard solutions of DON and D3G with concentrations of 1 mg L−1 were prepared in methanol/water (1 + 1, v/ v) and used as analytical standards.
Into each of the three vials containing 5 mg of DON, 10 mL from the solution B was injected for the reconstitution of DON.
Burton, though, has his own solution for the Dons' monetary ills.
Parties frequently don a hairshirt and promote solutions which may be electorally popular in the short term, but which in the longer term damage themselves and their rivals.
For overzealous researchers like Ms. Brooke, the solution probably lies in donning a proverbial fedora and playing it cool.
