We also incubated a series of commercially available epoxide hydrolases with DON under conditions recommended by the manufacturers but did not detect any transformation activity (unpublished results).
Motion segments were divided into discrete steps to facilitate statistical comparison of data between different conditions (DON and DOFF).
In absolute GH translation, the ABD motion segment (X, Y and Z axes) did not significantly differ between DON and DOFF conditions at the abduction range of ABD30° to ABD150° [Figure 5a, b and c] (Huynh-Feldt within subject effect P values 0.87, 0.88 and 0.92, respectively).
Similarly, in the ATM motion segment, the absolute GH translation components (X, Y and Z) did not differ significantly between DON and DOFF conditions at the ATM range of ABERMAX to ABER45° [Figure 6a, b, c] (Huynh-Feldt within subject effect P values 0.62, 0.75 and 0.85 respectively).
Microbial strains that de-epoxidize DON under anaerobic conditions have been isolated from animal digestive system.
The initial experiments focused on the stability/degradation of DON under hydrolytic conditions and the ability to release DON from the modified forms.
The possibility of de-epoxidation of DON under aerobic conditions may also be inferred from the study reported by Guan et al. (2009), who investigated the transformation of DON by fish digesta.
For the application of DON-degrading bacteria for detoxification in wheat fields, the bacterial candidates must have the capability of degrading DON under aerobic conditions; however, information about aerobic bacteria able to degrade DON has been limited.
Under the tested conditions, DON was not released from D3G, 3ADON, or 15ADON after hydrolysis and thus none of the published methods were able to cleave the modified forms of DON.
The partial 16S rRNA sequence of one of the bacterial isolates from chicken digesta that detoxify DON under anaerobic conditions shared 99% similarity to the 16S sequence of Bacillus arbutinivorans (Yu et al. 2010).
