Urinary DON concentrations (nanograms DON per milliliter of urine) were converted to urinary DON amounts (micrograms DON per day) based on 24-hr urine volume.
The results revealed high DON amounts in glumes and caryopses (167 ± 43 ppb and 152 ± 19 ppb).
When strain WSN05-2 was inoculated onto wheat grains treated with DON, the amount of DON on wheat grains decreased by about 90% in 7 days (Fig. 7).
Sample preparation for the published indirect methods for the determination of DON and the total amount of DON after acidic hydrolysis with trichloroacetic acid [ 13], trifluoroacetic acid [ 14], and trifluoromethanesulfonic acid [ 15] was performed.
It has also been demonstrated that treatment with the CTPsyn inhibitor, 6-Diazo-5-oxo-L-norleucine (DON), reduced the amount of CTPsyn tetramerisation in E. coli, which suggests that the tetramer form of the enzyme may be excluded from cytoophidia (Robertson, 1995).
While the latter is currently donning a tremendous amount of stage makeup and multiple wigs for Wicked, the former is gracing a more intimate stage, that of 54 Below.
The maximum amount of DON is produced by F. graminearum at 0.98 aw while Optimum DON production by Fusarium spp. is at pH of 7.5 (Comerio et al. 1999).
We measured the amount of DON in glumes and caryopses using an enzyme-linked immunosorbent assay for DON detection to confirm that optical detection of GFP fluorescence of TRI5prom:: GFP reporter strain results in a detectable production of trichothecenes.
This large amount of DON would likely constitute an important source of available nitrogen for plants when inorganic nitrogen could not meet the requirements for plant growth [30], [31].
The growth of WSN05-2 was directly proportional to the amount of DON in the medium.
