We modified the negative-dominance assay based on the λ cI repressor system developed by Zeng et al. [19] to detect protein-protein interactions.
In this study, we utilize λcI repressor LTTR fusions in a negative-dominance assay [19] to examine whether LTTRs from E. coli form promiscuous heterotypic interactions.
The phenotypic assay indicated dominance for bud induction and additive effects for both the percentage of explants with shoots and the number of regenerated shoots per explant.
Evidence for the distinction has primarily come from outcome devaluation studies, in which the sensitivity of a previously learned behavior to motivational change is used to assay the dominance of habits versus goal-directed actions.
10.7554/eLife.03830.007 Figure 5. Assaying the dominance of drug-susceptible genomes via the MOI-dependent detection of drug-resistance.
The tube test is a very sensitive assay for social dominance, and our results reflect an abnormal social interaction.
Due to this dominance of t003 and t008, these assays provide distinct utility within Luxembourg.
Thus, the superior DNA binding affinity of hPolη likely contributes to its dominance within the four enzyme combination assays.
Most PPIs in these databases have been identified using complementation assays, reflecting the continuing dominance of Y2H techniques for high-throughput PPIs determination.
Dominance (scored only in 'between condition' assays) was determined as repeated lunges by one fly followed by retreat of the other to the edge of the cup or off the cup altogether (Alekseyenko et al., 2014).
Similarly, we observed dominance of Hoxa13 over Hoxc11 in the same assay.
