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Carbohydrate-active enzyme domains assigned by the CAZy database (Cantarel et al. 2009) were searched for in the predicted protein sequences (Rho et al. 2010; Zhu et al. 2010) using HMMER 3.0 software (Finn et al. 2011) and the Pfam 26.0 database (Punta el al. 2012), with a cutoff of E ≤ 1e-4.
Table S3 shows the putative functions or domains assigned to 90 unigene-encoded proteins.
Moreover, as more protein structures are determined and more domains assigned the repertoire of domains that can be mapped to a given genome will also increase.
Phylogenetic analysis based the amino acid sequences of catalytic domains assigned these chitinase and chitinase-like proteins into eight separate groups (I VIII) (Figure 1).
Additional GO terms could be annotated to transcripts using InterPro protein domains assigned through InterProScan.
Table 3 shows the top 10 most frequently occurring protein families and functional domains assigned to our cDNA library.
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All ORFs were analyzed with the HMMer program (29) and assigned to PFAM families or domains, leading to 5822 domain assignments to PFAM-A, 1446 domains to PFAM-B and 1255 ORFs with no domain assigned.
Out of 14,625 single-spannings, 3516 sequences have at least one domain assigned, and no domains were assigned to 7850, with the remaining 3259 with less reliable assignment.
The Commerce Department announced yesterday that it had signed a contract with NeuStar Inc. to manage the database of Internet addresses ending in.us, which was previously administered by VeriSign Inc. The.us suffix is the country code top-level domain assigned to the United States, which has been used primarily by state and local governments and schools.
We also found that uncertainties originating from modeling approaches greatly depend on the types of hydrological structures and heterogeneity of hydraulic conductivity values in the domain assigned by modelers.
DMAR units translate and filter requests according to the protection domain assigned to the emitter device.
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