Previously, it was reported that the C-terminal 101 132 residues of Chlamydia trachomatis IncE interact with SNX5 and SNX6's PX domain.27 We found that a shorter fragment of IncE (109 132) retained the ability to bind to the PX domain of SNX5 (PX5, Supplementary Figure S1A).
Two of the antibodies, HPA002110 and CL0308, bind to epitopes within the extracellular domain of PODXL (Supplementary Figure 1A), whereas the epitope specificity for 4F10 is unknown.
The C-terminal end of Tuberin shares significant sequence similarity with the catalytic, but not the dimerisation domain of Rap1GAP (Supplementary Figure S1).
We probed 9,000 16-amino-acid-long 16-amino-acid-long 16-amino-acid-long 16-amino-acid-longigand sequence (peptidemmobilized on glasequences (Jerini SH3-domain profiler detector peptide arrays) using a Cy5-labelled recombinant SH3 domain of PSD95 (supplementary Table S1 online).
The mechanism for CXCR2 inhibition was pinpointed to cleavage of the N-terminal domain of CXCR2 (Supplementary Figure 4), a region that is necessary for proper chemokine binding and subsequent receptor activation.
As mentioned above, in theory, expression of exons 1 3 mRNA (encoding the first PDZ domain of GRASP65, supplementary material Fig. S2) (Truschel et al., 2011) should not be affected (Fig. 1A, targeted gene).
In contrast, 75% (67/90) of vertebrate-specific pairs involve at least one domain of metazoan origin (supplementary fig. S6 and spreadsheets S19 S20, Supplementary Material online).
For example, E. coli K12 harbors five EI-encoding and six HPr-encoding paralogs, either as single polypeptides or as domains of MTPs (Supplementary Table 2).
Our sequence alignments suggest that Tah1 is most similar to the TPR2b-domain of Hop/Sti1 (Supplementary Figure S1 at http://www.BiochemJ.org/bj/413/bj4130261add.htm), which influences the binding of Hop to both Hsp70 and Hsp90 [ 39].
No such interaction was seen between Aβ and the wild-type Val66 pro-domain of BDNF (Supplementary Material, Fig. S4A and B, nine paired experiments, n = 3 preparations of Aβ).
OPA1 preferentially interacts with full-length LRRK2 but not with isolated domains of LRRK2 (Supplementary Material, Fig. S1A), suggesting that the intact full-length protein is required for this interaction.
