Exact(5)
The transmission delay in the backbone between any two domain entries is assumed to be a small value δ, since the transmission delay in the fiber backbone is very low.
Protein domain and boundary information for each of the 11,128 proteins in the dataset was determined by sequence similarity search against domain entries in Structural Classifications of Proteins (SCOP) version 1.69 [32], at the e-value cut-off of 10−3.
Conversely, more general InterPro family and domain entries may be annotated with a more general GO term, subject to meeting evidence requirements.
The cluster file was loaded onto Treeview (version 1.60; http://rana.lbl.gov/EisenSoftware.htm) to generate a 2D branched-tree image including the phylogenic order of proteins and domain entries.
More specific family or domain entries, located at the leaf nodes of InterPro hierarchies (and which therefore might only describe a few well-characterized proteins) may be annotated with a correspondingly specific GO term.
Similar(54)
About 25% of UniSeqs matched a protein domain entry in the Pfam database [ 42].
The PFAM-A seed alignments (7) provided a suitable framework for establishing a database that comprised a catalogue of the evolutionary rate at codon sites for each protein domain entry.
HECT amino acid sequences were retrieved with a HMMER search, using the HMM profile of the Pfam HECT domain entry (PF00632) as a query, the default parameters and an inclusive E value of 0.05.
If we assume that after EGFR dimer activation domain entry is a random partition process, then the domain occupancy distribution will be a Poissonian aggregate distribution and the equilibrium distribution between domain and nondomain sites will be given by a simple linear partition function.
Additionally the PS01031 domain containing entries of rice were searched at (using taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01031).
Four decoy structures were used: profilin IB (PDB entry 1ACF, 125 residues), ketosteroid isomerase (PDB entry 3SED, 126 residues), a VH domain (PDB entry 1T2J, 116 residues), and human bromodomain (PDB entry 3HMF, 118 residues).
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