Those assessments, ideally, can be shown to correlate well with domain-competent or expert human assessment.
Three of these regulatory mutants, in which the linker was inserted in the N-terminal globular domain, were competent for the translocation of a cytotoxin, ExoU, into eukaryotic host cells.
These findings are supported by previous studies demonstrating that R42P and other mutants in the Ubl domain are competent for enhancing mitophagy (19, 21– 23); however, Lee et al. (20) suggested that the R42P mutant was inactive.
Structure-directed functional studies show that authentic MAL regulation requires that each of the three RPEL motifs in the N-terminal regulatory domain be competent to bind G-actin.
It has been recently demonstrated, however, that ErbB3 not only has kinase activity but its intracellular domain is competent to bind ATP and capable of catalysing auto-phosphorylation, a concept that is still being debated (Shi et al, 2010).
Previous studies have shown that the truncated versions of Smo lacking either CRD or the C-terminal domain remain competent to bind cyclopamine and other cyclopamine-competitive ligands, consistent with these molecules interacting with the 7TM segment (Chen et al., 2002a; Wang et al., 2013).
We speculate that only oligomeric forms of PAPC transmembrane domains are competent for its interactions with FLRT3 transmembrane domain.
We report a strategy to engineer an additional active site for human lysozyme, grafted the entire human lysozyme exon 2, which encodes the catalytically competent domain, into the gene at a position corresponding to an exposed loop region in the translated protein.
Ig-like domain mutants were competent of PA binding (Fig 7F), indicating that even if the Ig-like domain did not fold properly, the vWA domain did.
In cell extracts, the UBXN7 mutant lacking the UBA domain is fully competent in interacting with cullins, thus ruling out the involvement of this domain in cullin-binding.
The plateau in the time-course studies indicates that not all structures formed by the fifth domain provide kinetically competent folds for DNA repair.
