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This domain assignment helps assign catalytic residues to each family.
The Saccharomycotina-specific cluster contains the CFEM motif, but narrowly misses the threshold for InterPro domain assignment and has no GO terms assigned.
Based on our domain assignment in the 60S dimer, it is likely domain I of Sdo1p.
The performance of four automatic domain assignment methods, DomainParser, NCBI, PDP and PUU, is carefully analyzed using this broad spectrum of topology combinations and knowledge of rules and assumptions built into each algorithm.
The fragmentation based domain [40] is a simple method of domain assignment where all fragments on the query being present in the training set results in an 'in domain' result and new fragments on the query result in an 'out of domain' result.
Moreover, after domain assignment only the presence and absence of domains are evaluated in domain trees and domain combination trees.
The domain assignment provided by the SUPERFAMILY database gives the position and length of each domain within a given protein.
In some cases, domain assignment or verification involved manual comparison to the predicted domains of the proteins' orthologs listed in Homologene.
We used our 8,000 lowest-energy sequences as queries against the SUPERFAMILY library; significant hits were returned with the corresponding E-value and domain assignment.
The divergence of the sequences even results in different domain assignment in these two kinases: domain GCC is detected in ENSP00000350896 but not in ENSP00000281821.
Nevertheless, the current genome-wide domain assignment data are copious and significant enough to make evolutionary arguments, such as reconstructing species phylogenies based on protein domain content [5].
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