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Differences in vertebrate community structure between the prairie dog grasslands and the shrublands were expected.
Importantly, the prairie dog grasslands harbored many more priority conservation species compared to the shrublands.
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Little is known about how unique these prairie dog grassland communities are, how fast land degradation can impact biodiversity, and what the main conservation lessons are.
The prairie dog colony grasslands and the shrublands had differences in vertebrate species richness, composition, and abundance in the four vertebrate classes evaluated.
The small mammal trapping grids established on the prairie dog colony grasslands in 2000 2002 were in a different location than those established in 1992 1996 to accommodate different sampling designs.
For example, although the mesquite shrublands were richer in bird species than the prairie dog colony grasslands, as would be predicted for structurally more complex habitats [33], [34], [35], this result was the opposite from previous research in the area where bird species were three-times more rich in the grasslands than in the shrublands [36].
Our research also demonstrates that prairie dogs engineer grasslands by clipping shrubs, converting an invading shrubland back to open grassland.
Lagomorphs and carnivores were sampled along all available roads within the shrubland and prairie dog colony grassland study sites from 1994 1996 and 2000 2002 (Figure 2 and Table S3).
Consequently, the presence of prairie dogs in these grasslands increases grassland landscape heterogeneity at large spatial scales, potentially enhancing beta diversity.
While our previous studies have compared vertebrate diversity in prairie dog colonies and adjacent grasslands in this area between 1992 1996 [55], [56], [56], the grassland without prairie dogs is a transient vegetation type.
Differences in landscape structure between prairie dog towns and unmodified grasslands had a significant effect on the movement of the darkling beetle, Eleodes hispilabris Say.
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