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Ultimately, we ask ourselves: does this manuscript constitute a substantial step towards a clear answer to an important global health question?
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No such study has been conducted within Pakistan that studies the topic done in this manuscript.
We believe that these are all good reasons to compare approaches in applied settings, as we do in this manuscript.
In doing so, this manuscript aims to: (1) deepen the background on local climate to better understand the public health challenges related to climate and climate change and (2) document some of the climate data resources available to those studying health impacts in the LMICs.
Unfortunately, the authors were not asked to do this and the manuscript was inadvertently published with the patent citation included.
Only later in the manuscript did this become clear.
We completely agree and have done this in the revised manuscript.
We have done this and modified the manuscript appropriately (see new Figure 4).
My main concern is that this manuscript does not do justice to the work others have done regarding the co-transfer of genes: Phylogenetic profiling [ 1] is an approach to identify the function of genes due to their frequent co-occurrence in genomes.
It would be really really nice if this were established in this paper but I frankly do not think that this manuscript actually does anything of the sort.
The questions that we try to answer in this manuscript are: does TGF- β1 work indiscriminately on all cell subpopulations or only on specific cell subpopulations?
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com