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In addition, PIPKIγ_i5 did not co-immunoprecipitate with talin1, talin2 or α-adaptin (results not shown), confirming that, although the C-terminus of PIPKIγ_i5 contains this similar sequence, it does not target to the same cellular domains and probably cannot functionally compensate for PIPKIγ_i2.
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Surprisingly, we found that the amphipathic helix alone (850 858) did not target to the PM (Fig. 5A, Bud3p-M9).
As expected, Bud3p-N/M2 and Bud3p-N/M6 that could partially restore axial budding localized to the bud neck, whereas Bud3p-N/M4 and Bud3p-M16 that could not restore axial budding did not target to the bud neck (Fig. 7B, left panel).
As expected, overexpression of NES TG2 and NN TG2 (which do not target to the nucleus) increased PGC-1α and cytochrome c expression in Q111 cells (Fig 2D).
Previous phylogenetic observations of nucleomorph-encoded proteins, have led to speculation that the nucleomorph genomes are extraordinarily divergent, however these studies have been made of proteins that do not target to the plastid.
SI: A key criticism of the past incarnation of PEPFAR is that it frequently didn't target to the demographic of the epidemic.
Importantly, this ubiquitin modification is predominantly monoubiquitination, which does not target Ro52 to the proteasome for degradation.
Analysis of the Map-Cdc42 signaling cascade showed that EBP50 does not target Map to cell membrane receptors.
w Country's vitamin A supplementation programme does not target children up to 59 months of age.
The case study with octopus reaching movements, of course, does not target as a final goal to replicate reaching movements in surgery.
However, current delivery systems for IBD do not target drugs to the site of inflammation, which leads to frequent dosing and potentially severe side effects that can adversely impact patients' adherence to medication.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com