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Reviewer 1: The authors use point mutations in GFP-tagged CENP-M to suggest that 'CENP-M and CENP-I are mutually required for kinetochore localization', as mutant CENP-M does not localize to kinetochores or rescue CENP-I localization.
However, this FAM-Aβ42 does not localize into acidic organelles, as evidenced by low co-localization with LysoTracker.
However, this does not necessary mean that the protein does not localize in mitochondria.
These data also raise the question why hCAREx8 does not localize to the basolateral surface.
Furthermore, matrix present in HIV particles does not localize to the nucleus [19].
The Bud3p (1 858) mutant does not localize to the bud neck (Fig. 7B).
CHD1 does not localize to centromeres in either S2 cells or developing fly embryos.
However, HCNCp has multiple "CxC" motifs rarely found in VSPs, and does not localize to the trophozoite plasmalemma.
The sGCΔN mutant has the opposite properties: it produces cGMP but does not localize to the leading edge.
These findings are in line with that fact that p19 does not localize to the plasma membrane (Figure 1A).
Consistent with this, human Plk1 does not localize to the central spindle when heterologously expressed in Drosophila cells and embryos [23].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com