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Hexahistidine tagged PilA does not form detectable pili but supports transformability independent of functional PilT 78.
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We also found that most transcripts whose levels were altered by in vivo manipulation of RNase H levels did not form detectable R-loops, suggesting that prolonged manipulation of R-loop levels could indirectly alter the transcriptome.
Although they did not form detectable tumors in nude mice, HPKII cells did form benign cysts [12], [13].
In contrast, the C-terminal coiled-coil domains (GR1 and GR2) of the GABAB-R1 and GABAB-R2 receptors do not form detectable homodimers either in vivo or in vitro.
In contrast to control cells siCSN1-LiSa-2 cells did not form detectable lipid droplets during 22 days (Fig. 3C).
Further, Epo did not form detectable light chain of plasmin in a 3-h incubation with plasminogen, which however was discernible with the Pla-expressing bacteria.
Although CHD4 did not form detectable foci after cell exposure to ionizing radiation (IR) or genotoxic drugs (data not shown), CHD4 displayed enhanced resistance to detergent extraction very early on after treating cells with the DNA-damaging agent H2O2 (Supplementary Figure S1E), consistent with CHD4 rapid recruitment to damaged chromatin.
In this case, the detectable range of temperature was limited because PNIPAm does not form a gel in water below its lower critical solution temperature of 32 °C.
Mouse embryos lacking SRF die before gastrulation and do not form any detectable mesoderm [ 33, 34].
Native gel electrophoresis was used to confirm that these oligonucleotides do not form any detectable secondary structure.
R. equi 103+ or 103+/ kasA grown as above in BHI for 18 h were added per well at 1.5 × 10 bacteria and the cultures in a total volume of 200 μl per well were left standing in a humidified incubator at 30°C for 16 h, centrifuged at 1200 g for 15 min. MIC was defined as the minimal antibiotic concentration at which bacteria did not form any detectable pellet.
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