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The effect of processing on the tertiary structure of collagen is assessed by monitoring the wavenumber of the N H stretching vibration peak using Fourier transform infra-red spectroscopy and it is found that processing does not denature the collagen.
PFO is a much milder detergent than SDS; it coats proteins with negative charge but does not denature them.
Even when the sample still contained genomic DNA, DNA amplification is unlikely to occur in the OSNA assay because the RT-LAMP step is carried out at 65 °C, a temperature at which genomic DNA typically does not denature.
Contrary to Nozaki and Tanford's earlier conclusions, our published data show that urea does not denature proteins by favorable interactions with nonpolar side chains; rather it is through its favorable interactions with the peptide backbone that urea is such a good denaturant (3, 8, 27− 27−.
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It doesn't denature proteins as harshly as direct heat methods, so the meat stays much more tender than the same cut cooked over direct heat.
The analyses of biodegradation characteristics in bio-hydrogen producing process implied that ultrasound did not denature the digestion sludge but just improved its biodegradation efficiency.
The molar ratio polyanion/protein corresponding to onset of the second stage increased with elongation of chains and eventually it disappeared for highly polymerized polyanions which did not denature the protein.
While we did not denature the proteins before Bradford analysis, our protocol does include a preclear step to remove cellular debris.
As a consequence of the small size the binders do not denature, and they can be stored in the lyophilized state without loss of activity.
We find that a major factor that can influence such regions are intervals of high C + G content that do not denature efficiently under routinely used conditions.
Excess free biotin was used as a 'sink' to prevent rebinding of displaced streptavidin and 0.1% SDS was used to stop translocation, conditions that do not denature streptavidin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com