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Importantly, mouse tpt1 mRNA does not contain the sequence that is targeted by the Xenopus antisense oligos.
Although the isolated cDNA 8B6 does not contain the sequence encoding the N-terminal amino acids of GALT3, peptides from whole GALT3 were included in the peptide screening.
As anticipated, BAP-24 on it own is not a substrate for the BoNT/A LC because it does not contain the sequence recognized by the active site.
An amplicon including an intron is quite long and usually results in an incomplete transcript that does not contain the sequence for the reverse primer, thereby preventing further amplification.
In the case of Slo2.2 KO, deletion of exon 11 (encoding amino acids 285 354 of Slo2.2) causes a frame-shift such that the predicted residual Slo2.2(1–252) protein does not contain the sequence recognized by the NeuroMab anti-Slo2.2 antibody.
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* similar results have been obtained for a smaller dataset that does not contain the sequences from the coronate Linuche unguiculata, the tube anemone Ceriantheopsis americanus and the blue octocoral Heliopora coerulea.
The sequences did not contain the sequence of the Pdcd2 constitutive exons (Fig. 2).
However, these datasets do not contain the sequence of all genes or that of intergenic regions.
Thus, the PCR primers did not contain the sequence to be deleted and amplified outward from the deletion point.
In accordance, their genomes do not contain the sequence corresponding to the enzyme responsible for cortisol synthesis (11b-hydroxylase) and in general classical steroids except estradiol are rarely found in lamprey.
Alternatively, the option for alternative transcript initiation may provide a further layer of regulatory control: The short transcripts do not contain the sequences previously implicated in FVIII transcriptional silencing [45], but it is recognised that minigene insertion into FVIII intron 1 improves in vivo production of FVIII [46].
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