The changes in N-glycan structure occurring in the ER did not affect further modification of the Chp glycans in the Golgi apparatus, and the results strongly suggested the presence of a regulation of N-glycan processing based on the 3-dimensional structure of the backbone polypeptide [12], [13].
Tenfold increased KDO concentration did not affect further the receptor expression.
Notably, during the following two days (d1, d2) cellular reorganization continued in the tumor spheroids resulting in moderate compaction and associated significant volume reduction, which did not affect further experiments.
This does not affect making further mutants, as we have successfully inactivated the adc gene in strain SMB009 using pMTL007.
Although some authors consider that the endocanalar zone does not affect adhesion, further studies have revealed differences [ 25, 26].
Our findings suggest that in vitro transfection of immature DCs with adenovirus does not affect its further development into mature DCs.
In addition, when CHX-based counterselection is applied to select for FZO1 loss, subsequent removal of CHX does not affect the further proliferation of fzo1 ∆ aac2 ∆ PDR1 -249 cells, indicating that any CHX-driven modulation of the PDR pathway (Thakur et al. 2008) is not relevant to the genetic interaction that we have uncovered.
The simulation showing that H30 expression does not affect the cAMP microdomain further reinforces the previous results and confirms the experimentalists' assertion that the cAMP microdomain is not an artifact of either H30 expression or unbalanced concentrations of the FRET sensor.
In the present experimental model, the impact to the dorsal aspect of the spinal cord compresses and ruptures a number of these small vascular units underneath and around the impactor tip, but probably does not affect vascular units located further away.
Therefore TETA treatment at the dosage employed does not affect copper transport, and further data from TETA-treated non-diabetic controls are not required for interpretation of results of the current study.
