To explore the possible involvement of Bak in breast cancer with reference to clinical factors, the representative pattern of the Bak expression, measured by RT-PCR, is shown in Figures 1 and 2. The expression levels were quantified by gel documentation program and normalized against the internal control GAPDH expression.
Amplicons were quantified using Alpha DigiDoc gel documentation and image analysis system (Alpha Innotech, USA).
The signal intensities of the respective bands were quantified with a gel documentation system.
Western blots were quantified by applying a Kodak gel documentation system (model 1D 3.5) and standardized with loading control.
Protein levels were quantified using densitometry on an Eagle Eye gel documentation system (Stratagene, La Jolla, CA, USA).
Everything can be quantified, measured and analyzed.
The expression level of Bak gene was quantified by gel documentation and normalized against the internal control GAPDH expression [ 13, 14].
52 Blots were developed using enhanced chemiluminescence (ECL) (Pierce, Rockford, Illinois, USA) and signal intensity was quantified by densitometry using the Electrophoresis Documentation and Analysis System (EDAS) 120 system (Kodak, Rochester, New York, USA).
Expression was quantified by an Alpha Imager 2000 documentation and analysis system (Alpha Innotech Corporation, San Leandro, CA, USA).
The relative intensity of bands was quantified by the ChemiDoc CRS gel documentation system and Quantity One software from BioRad (Hercules, CA, USA).
