Exact(1)
The guidance covers a wide range of processes involved in stem cell banking, including procurement of cell lines, cell banking procedures and documentation, cell banking quality control, and the process of releasing cell banks.
Similar(59)
For documentation of cell morphology, images from unfixed cells were obtained using an Axiovert 10 microscope (Zeiss, Oberkochen, Germany) and a DS-5 M-L1 digital sight camera system (Nikon, Düsseldorf, Germany).
For documentation of cell morphology, images from unfixed cells were obtained using an Axiovert 10 microscope (Zeiss, Oberkochen, Germany) and a DS-5M-L1 digital sight camera system (Nikon, Düsseldorf, Germany).
Critical barriers to successful implementation of stem-cell-based therapy for regenerative medicine include documentation of cell tracking studies during pre-clinical trials.
In comparing our detailed documentation of cell lineages of P. mammillata with available information on cell lineages in other ascidian species, we found more similarities to the described cell lineages of C. intestinalis [ 27, 44– 46] and H. roretzi [ 42, 43] than to the only publication of a cell lineage of P. mammillata itself [ 48].
Our detailed documentation of cell lineages of this very species shows more similarities to the described cell lineages of C. intestinalis and H. roretzi than to the description of the cell lineage of P. mammillata [ 51].
Photo documentation and cell counting by using a counting chamber was performed at every passage.
Photo documentation and cell counting by using a Neubauer counting chamber was performed at every passage.
The density of the individual bands was measured on an AlphaImager™ gel documentation system (Cell Biosciences).
Documentation of cell types and relative amount of each type by visual inspection of two-dimensional slides of adjacent tissue as done here was feasible and easy, but utmost important as the variability of cell content was high.
Chemoluminescence was analyzed using a Fluor Chem Q gel documentation system (Cell Biosciences, Victoria, Australia).
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