The intensity of the bands in the gel was visualized by use of the SynGene gel documentation system and analyzed with use of Genetools (Syngene, MD, USA).
Images were captured by Alpha Image 950 documentation system and analyzed by NIH Image version 1.62 (Alpha Innotech, San Leandro, CA).
Data on technical issues of reagents preparation, smearing, staining, reading of the microscope slides, reporting pattern of the findings and recoding and documentation were also collected and analyzed as per the standard set by WHO.
One-fifth of each PCR reaction was run on a 1.5 2% agarose gel in 1×TBE buffer (45 mM Tris/borate/1 mM EDTA); the gel was documented using the AlphaImager 2200 gel documentation system (Alpha Innotech, San Leandro, CA) and analyzed using the publicly available NIH ImageJ 1.33u program [ 52].
"There are interrogations taking place, there's documentation being found and discovered and analyzed and translated, so that each day we learn more and know more as more address books are found and phone books are found and computer hard drives are found," he said.
Then bands were visualized by ethidium staining for 10 min and analyzed by documentation equipment (Syngene, Cambridge, UK), gel scanning software (Syngene, GeneTools) and SigmaPlot version 12.3.
The amplification products were resolved in 2% agarose gel, documented in Gel Documentation System (Alpha Innotech Corporation, USA) and analyzed by densitometry (using ChemiImager Software) for Integrated Density Values IDVV).
The DNA and its fragments on gel were detected by treatment with ethidium bromide and the florescence produced by UV light was photographed and analyzed using gel documentation system (G box, syngene, UK).
Upon conclusion of the interviews, both forms of documentation (audio and written) were reviewed and analyzed to facilitate preparation of a comprehensive report.
Gels were recorded and analyzed using a gel documentation system (DigiDoc-It Im aging System, UVP).
The gel was stained with ethidium bromide and analyzed using a gel documentation system [ 48].
