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However, most of them share a short, degenerate "RVxF -type docking motif.
We looked at sequences of virus proteins without a docking motif coincident with the spatial position of the standard motifs.
These results also suggest that this combinatorial PP1c docking motif is potentially a molecular tool for drug design.
We also considered whether or not an infrequently observed MAPK docking motif among human proteins could serve as an HIV strain-independent MAPK docking site.
This similar biochemical foundation explained why the candidate docking motif Dc was coincident with Da or Db in the HIV proteome.
Given that AIF562 571 shares a similar FxxR/KxR/K PP1c docking motif, we hypothesized that it might constitute a cell penetrating and cell death sequence.
The ELM Resource describes one version of the D-site (Da), while the current literature contains another frequently observed MAPK docking motif (Db), with a regular expression similar, but not identical, to that of Da [12].
Taking clues from these perturbations, we designed a new regular expression for a candidate MAPK docking motif along HIV proteins (Table 1), and represented this motif with the symbol Dc.
The docking motif containing Lys235 in the StAR sequence and Asp319 in the ERK sequence constitute the closest interaction, separated by a short distance, approximately 11 Å (Fig. 6).
We have previously shown that calcium may regulate the protein-protein interaction of ERK1/2 [56], [56], which may be mediated through the cytosolic retention sequence (CRS)/common docking motif (CD) of these ERKs.
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Interestingly, this peptide contains a potential FxxR/KxR/K PP1-docking motif suggesting that binding of this peptide to PP1c may be involved in the apoptotic process.
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