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We compared biomass and glutathione producing capacities of H. polymorpha DL-1 wild-type strain under different dissolved oxygen (DO) saturation conditions, DO-stat 30% and DO-stat 60%, provided in fed-batch cultures in glucose minimal medium (see Materials and Methods).
DO-stat 30% (mode I) was chosen to create reduced oxygen concentration and DO-stat 60% (mode II) was realized for maintenance of oxygen saturation condition.
It has been done two modes of glucose feeding, using DO-set points 30% and 60% (DO-stat 30% and DO-stat 60%), both modes were based on dual control algorithm.
Nevertheless, we did not observe strong increase of TIG concentration in methanol-feeding wild type cells (DO-stat 60%) relative to that of glucose-feeding cells (DO-stat 60%) (Table 2).
Dual control of methanol feeding with DO-set point 60% (DO-stat 60%) provided permanent biomass growth.
Methanol fed-batch fermentations study was carried out only under DO-stat 60% that provides the highest biomass yield of H. polymorpha compared to DO-stat 30% [ 33, 34].
Unexpectedly, enhanced oxygen supply (DO-stat 60%) caused reduction of biomass yield of fed-batch cultivated wild-type cells in glucose-containing minimal medium.
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