However, these new devices challenge the classical way to do spike sorting.
Approximately 16 +/− 4 h after inoculation of the fermenter, a DO spike was observed due to the depletion of glycerol within the media.
After observation of the DO spike, a bolus of 50% sorbitol (~9.0 g/L) was added to the fermenter over a 30 min period.
When a steady DO rate and fast DO spike time was obtained, indicative of adaptation to methanol utilization, the feed rate was increased to 3.96 ml/h for the remainder of the fermentation.
When the initially added glycerol was consumed completely (indicated by a DO spike after 13.5 h), a glycerol feed (50% glycerol supplemented with 12 mL L−1 Ptracerace salts) was started.
Know that women can and do spike as well.
For a pixie do, spike your hair slightly and place the headband three inches back from your hairline.
If somebody did spike his drink, he is presumably still furious, no?
After a continual drop in the growth of those on DSP from 2002 to 2007, the numbers did spike significantly until 2010.
