In the mid 20th century, the computer giant IBM's entire business relied on paper, with its famous punch cards ("Do not fold, spindle or mutilate") being used to record units of information in the form of code.
As the primary means of writing programs, storing data, running a payroll and issuing checks, they became a symbol of big-company automation and government bureaucracy ("Do not fold, spindle or mutilate").
He saw the seeds of a new future in campus rebellions, the civil rights movement and even a popular button that said, "I am a human being; do not mutilate, spindle, or tear".
One caution to parents: Don't fold, spindle or mutilate before the children get their turn.
Knocking down these proteins appears to suppress SAC activation by K5I, but not by taxol or nocodazole, yet they do not rescue spindle bipolarity, at least by the criterion of promoting cytokinesis.
Insert New Comment, "This is THE MagicNumber!!!!! Do NOT change, spindle or mutilate, PLEASE!
Insert New Comment, "©CG: This is THE MagicNumber!!!!! Do NOT change, spindle or mutilate, PLEASE!
Similar to the situation in Drosophila, non-phosphorylatable mutants of Eg5 do not localize to spindle microtubules when overexpressed in cultured human and Xenopus cells [7], [15].
Interphase fission yeast and animal cells do not have a spindle, which typifies cells in mitosis (Fig. 1, c g), but they have a cytoplasmic array of microtubules that are absent from mitotic cells.
These observations indicate that embryo extracts do not entirely preserve spindle morphology and function.
