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The SSU rRNA unaligned sequences were spliced into 12 blocks using conserved regions as a reference and, along with the LSU fragment, analyzed under direct optimization (DO) as implemented in the POY 4.0 program.
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Calculations were done as implemented in the SITES software [ 45].
GSEA analysis was done as implemented in the phenoTest package of Bioconductor.
We were able to meet our basic goals as well as get a good amount of extra work done, such as implementing wireless communication, which was originally a farfetched dream.
... But regarding the Affordable Care Act and the health insurance exchanges, you don't have to do anything". The ACA as implemented bears little resemblance to the fears that were once expressed in fierce opposition.
Finally, our IPW, as implemented, do not include lead measurements on nonparticipants and, therefore, assume that all effects of lead exposure on participation occur through measureable health effects or other conditions available in our data.
However, this program, as implemented, does not accomplish the intended goal.
Classification was done using Support Vector Machine classifiers as implemented in the WEKA machine learning package [ 27].
All registration steps were done using trilinear interpolation as implemented in FLIRT (Jenkinson and Smith 2001; FSL 4.1.4).
Current implementations of the binary (Bernoulli model) version of the scan statistic allow adjustment for categorical covariates only, and the M-statistic as implemented does not adjust for covariates at all (although allowing for categorical covariates via stratification would seem to be a straightforward extension of the existing method).
For multiple sequence alignment of the Ground and Ground-like domains MAFFT was used [see Additional file 3]; in case of GRD-1, GRD-2, and GRD-11 the four domains were extracted by hand and added to the alignment labeled with R1 to R4. Phylogenetic analysis of the alignment was done using Neighbor joining as implemented in ClustalX [ 36].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com