In 1999, 4.8% of 228 DNR-collected samples were positive for P. piscicida, whereas in 2000, 1.8% of 381 DNR-collected samples were positive.
Other rivers in which P. piscicida was detected in 2001 (DNR and waterman samples) included the Big Annemessex (n = 2), Manokin (n = 3), Choptank (n = 1), and Pocomoke (n = 1) rivers and Tangier Sound mainstem (n = 2).
Other rivers in which P. piscicida was detected in 2002 (DNR and waterman samples) include the Tangier Sound region (n = 15), Chesapeake Bay main-stem (n = 4), and Honga (n = 3), Nanticoke (n = 3), Choptank (n = 2), Little Choptank (n = 2), Manokin (n = 4), Pocomoke (n = 11), and Wicomico (n = 1) rivers.
XW, JMC and DNR were responsible for samples and genotyping for MCBCS.
In 2002, P. piscicida was detected in 7% of samples collected by DNR and in 2.6% of waterman-submitted samples, a variation that we again attribute to heavier sampling in the Chicamacomico/ Transquaking region by DNR (19 of a total of 27 positive water samples were obtained in this watershed; six waterman samples collected in this system were positive).
Samples collected by DNR staff during 1999 (n = 228), 2000 (n = 381), 2001 (n = 438), and 2002 (n = 387) were obtained from the lower eastern shore tributaries where the enrolled watermen worked.
There was no evidence of DNR orders in case records sampled from the GSF GP Practice or Heart Failure Community Matron services.
The Regional Ethical Review Board determined that, due to the population-based nature of the study, informed consent from study participants was not required (EPN-Dnr: 2011/1026-31/4) and collection of the samples for histology review and HPV-typing was also allowed (EPN-Dnr: 2012/1028/32).
CyA had no significant effect on Dnr accumulation in four of the mdr1-negative samples.
