Exact(4)
A modified CTAB (hexadecyltrimethylammonium bromide) protocol [ 58] was used, and the DNAs were further purified by two-rounds of phenol extractions.
All DNAs were further screened for DiGeorge and Williams abnormalities and those found with such anomalies were excluded from the study.
The extracted DNAs were further purified using the DNeasy Plant Mini Kit (QIAGEN) and treated with ATP-dependent DNase (TOYOBO) to remove linear double- or single-stranded DNA.
Ta annealing temperature To confirm genotype results 25%% of sample DNAs, randomly selected, were blindly analyzed in an external laboratory ARMS and H-ARMS PCR analysis (with a different thermal cycler; moreover, in both the laboratories, 5%% of DNAs were further tested by direct sequencing on both DNA strands).
Similar(56)
Systems capable of separating RNA from plasmid DNA were further analysed and applied to extract RNA from plasmid DNA out of a preconditioned cleared lysate.
Plasmid DNA were further purified using phenol-chloroform-isoamyl extraction and ethanol precipitated.
The immunoprecipitated DNA as well as the input DNA were further amplified using Sigma WGA2 kit.
The ChIP DNA and the input DNA were further sequenced using Illumina sequencing platform.
Purity of isolated genomic DNA was further assessed by electrophoresis in agarose gel (0.8%).
After removal of the oil phase, the amplified DNA was further purified with SPRIselectTM beads in accordance with the ThunderBolts Cancer Panel Manual RainDanceTM Technologiess).
The DNA was further cleaned using PowerClean Pro DNA Clean-Up Kit (MO Bio Laboratories, Cat. No. 12997-50) and used for the library preparation for subsequent sequencing.
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