The quantification of fungal biomass in infected B73 and derived NILs was based on the first standard curve (created from mixing with a constant amount of B73 DNA), while the quantification for DNA from infected Tx303 was based on the second standard curve.
All transcripts required for vegetative growth are derived from macronuclear DNA while the transcriptionally inert micronuclear DNA is organized into heterochromatin [ 20].
Of the DNA extraction methods tested, sonication did not produce high molecular weight DNA while the differential centrifugation method generated a DNA pool dominated by bacterial DNA and therefore not suitable for analysis of eukaryotes.
In this image, colored bars represent the bases that make up a strand of DNA, while the green circles represent methylation.
The assay was carried out through target-induced conformational change of hairpin DNA, while the signal derived from the formed silver nanoclusters on hairpin DNA probes.
The detection limit of the novel LAMP assay was determined to be as low as 1 fg of T. evansi DNA, while the reaction time for the test was only 30 min.
The Precellys extraction allowed the detection of both intracellular and superficial DNA, the DNeasy Blood & Tissue kit the specific detection of intracellular DNA, while the Instagene kit detected only superficial DNA.
The Supreme Court should take up this case, but there was no good reason for the chief justice to allow the police to continue collecting DNA while the case is on appeal.
In this system, the PP can be circularized after hybridization with the precisely-matched target DNA, while the stem of MB can be also opened by target DNA, resulting in hybridization with the circularized PP to generate a long tandem single-stranded DNA (ssDNA) product.
The compound with an ortho-chlorobenzene group at B ring exhibited the highest ability to inhibit ·OH-induced oxidation of DNA, while the compound with a meta-hydroxylbenzene group at B ring inhibited Cu2+/glutathione (GSH -induced oxidation of DNA efficiently.
Real-time PCR indicated that the Nitrobacter population decreased from 2.42×104 to 1.34×103 16S rRNA gene copies/ng DNA, while the quantity of denitrifiers obviously increased from 0 to 2.51×104 nosZ gene copies/ng DNA with an increasing C/N ratio.
