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This strand is then immobilized on a solid surface through a complementary DNA, which we term the 'surface strand' (Fig. 1a).
Announcements of that sort have been based on one of two types of tests: Y-DNA (which follows an unbroken line of males, popularly called the surname line) or mitochondrial DNA (which we inherit from our unbroken line of mothers).
Our work has demonstrated the successful combination of the operating principles of DNA-based nanomechanical device with the unique molecular recognition properties of DNA, which we believed could open an exciting avenue in the design and construction of easy-to-handle, cost-efficient, reliable and high efficient functional nanostructure.
Thankfully, our cells contain a further layer of protection, in the form of a variety of 'toolkits' that can repair damaged DNA (which we've discussed at length in this post).
The transcriptional silencing of Dnmt1 may be responsible for the widespread passive hypomethylation of genomic DNA which we detect on the example of pericentromeric repeat sequences.
One hypothesis is that these two looped states correspond to two different configurations of the Lac repressor molecule and its attendant DNA, which we will refer to as the "open" and "closed" configurations.
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In the present study, we used only high quality regions (quality value ≥ 25) of raw shotgun reads from three different sets of genomic DNAs, which we used for determining the draft genome sequence of C. intestinalis[ 1].
Furthermore, thermostable DNA ligase from Thermotoga maritima (Tma DNA ligase), which we previously reported, is employed to ligate the nick of the newly extended DNA strand, resulting in the formation of circular plasmid DNA as the amplification product that could directly be transformed.
The web of complexity associated with ncRNAs is aptly summarized in a figure, also used as the front cover of the book, which shows a proportional increase in the amount of non-coding DNA (aka "junk" DNA, which today we know codes for ncRNA) in the genome as we climb the evolutionary ladder toward more complex organisms.
This inflammation was due to the cytidine phosphate guanosine content of DNA, which, as we have previously shown, promotes both macrophage and polymorphonuclear leukocyte activation and production of proinflammatory cytokines.
We find that this measure of DNA concentration, which we call the Normalized Electrode Intensity, has a sharp transition at around 100 kHz associated with the onset of positive DEP.
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