Exact(8)
Systems capable of separating RNA from plasmid DNA were further analysed and applied to extract RNA from plasmid DNA out of a preconditioned cleared lysate.
Plasmid DNA were further purified using phenol-chloroform-isoamyl extraction and ethanol precipitated.
After isolation of RNA, traces of contaminating DNA were further eliminated by treating RNA samples with RNase-free DNase I (Ambion, Austin, TX) at 37°C for 20 min. Samples were either used immediately or stored at −80°C.
The immunoprecipitated DNA as well as the input DNA were further amplified using Sigma WGA2 kit.
The ChIP DNA and the input DNA were further sequenced using Illumina sequencing platform.
More importantly, as a result of flocculation, HCP and host DNA were further removed in the Protein A step to levels that meet the requirements of drug substance and thus reduce the impurities load to subsequent purification steps.
Similar(52)
The interaction of complex 1 with calf thymus DNA (CT DNA) was further investigated by absorption and fluorescence spectroscopic methods.
The DNA is further organized in nucleosomes which contain 146 basepairs of DNA and eight histone proteins (4 × 2 histone proteins).
The integrity and purity of extracted DNA was further assessed on the 1.0% agarose gel and measured using a Nanodrop spectrophotometer (Nanodrop 2000, Thermo Scientific, Japan).
The metal ion selectivity of the catalytic DNA was further improved using a 'negative selection' strategy where catalytic DNA that are selective for competing metal ions are discarded in the in vitro selection processes.
The DNA was further stored at 4°C.
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