Exact(7)
The large arrays of alpha satellite and gamma satellite DNA were enriched for both euchromatic and heterochromatic modifications, implying that some pericentromeric repeats have multiple chromatin characteristics.
The underlying sequence did not correlate with chromatin states, because both higher-order alpha-satellite DNA and noncentromeric DNA were enriched for modifications that define CEN chromatin, euchromatin, and heterochromatin.
We also observed that fragments with sizes in the order of tri-nucleosomal DNA were enriched for alpha repeats.
Size-selected fragments from genomic DNA were enriched for SSR content by using magnetic streptavidin beads and biotin-labeled CT, GT, AAG, and ATGT repeat oligonucleotides.
Size-selected fragments from genomic DNA were enriched for simple sequence repeats (SSRs) by using streptavidin-coated magnetic beads and biotin-labeled CT and GT repeat oligonucleotides.
In a second approach, also done at Ecogenics GmbH, size-selected fragments from mechanically sheared genomic DNA were enriched for SSR content by using magnetic streptavidin beads and biotin-labeled CT and GT repeat oligonucleotides.
Similar(53)
Methylated DNA was enriched from 0.5 to 2 µg of genomic DNA obtained from 9 MM, 3 MGUS and 3 NPC specimens (MethylaMiner™Methylated DNA Enrichment Kit; Life Technologies Corp., Carlsbad, CA, USA) according to the manufacturer's instructions.
The first comprised 104 hereditary cancer patients, whose DNA was enriched for target sequences using a custom-designed hybridization enrichment reagent capturing the exonic regions of 36 cancer-associated genes (Supp. Table S1).
454 sequencing was performed by Ecogenics (Zurich-Schlieren, Switzerland) as follows: DNA was enriched for two repeat motifs (CT and GT) representing all enrichable dinucleotide motifs, and sequenced on a GS FLX (Roche Applied Science, Indianapolis, Indiana, USA) after library construction.
Besides the inherent chirality, DNA is enriched by nitrogen and oxygen functional groups that are preferential to coordinate with transition metal ions, and its self-assembled structures, including the G-quadruplex, the i-motif, and the conventional Watson Crick duplex, etc., can be adjusted via different base pairings.
The remaining unbound target DNA is enriched in sequences absent in the subtracter DNA.
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