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A preferred site for rat liver topoisomerase breakage in single-stranded phi X174 DNA was located within a region of the DNA with the potential for duplex formation.
DNA was located on preparative gels using UV shadowing over fluorescent silica gel plates (EMD Chemicals 5715-7).
However, one of two variants of uncertain significance (due to inability to PCR amplify a gene product from matched normal DNA) was located in the kinase domain of HER2 (and784Cys) and has not been previously reported.
In contrast, almost all DNA was located in the cytoplasm, indicating that the cytosolic, chromatin-like, electron-dense material evident in TEM was definitely chromatin.
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Wozniak, M. A., Mee, A. P. & Itzhaki, R. F. Herpes simplex virus type 1 DNA is located within Alzheimer's disease amyloid plaques.
Although RNA molecules are synthesized in the cell nucleus, where the DNA is located, most of them are transported to the cytoplasm before they carry out their functions.
But when the correctly matching DNA is located, Cas9 undergoes a large structural change that releases this inhibition and triggers DNA cutting," said first author Samuel Sternberg, who recently received his Ph.D. at UC Berkeley.
A two phase system is discussed, in which the DNA is located in buffered solution whereas the hydrophobic porphyrins, responsible for the charge separation reaction, are located in the lipid bilayer of liposomes or supported lipid bilayers.
In the control sets (0 min incubation), most of the DNA is located in the head of the comet while cells subjected to longer treatment show increasing DNA damage and longer comet tails.
The fluorescent anti-PARP-1 staining outlines the perimeter of the S. solfataricus cells (Fig. 1a, bottom left; green), as does the membrane staining (Fig. 1a, top, right; red), while the DAPI-stained DNA is located in the cell centre (Fig. 1a, top middle; blue).
In eukaryotes, genomic DNA is located in the cell nucleus, along with small amounts in mitochondria and chloroplasts.
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