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However, it was only when this DNA was inserted into a sheep's egg from which the nucleus had been removed that Dolly's genes could produce another Dolly.
The DNA was inserted into the pET22b vector for expression.
PCR results demonstrated that the transformed DNA was inserted into the genomic DNA by the homologous recombination.
The stem-and-loop structure, which contains a strand complementary to the target DNA, was inserted into the G-quartet structure of the thrombin-inhibiting aptamer to disrupt the G-quartet structure through the hybridization of the target DNA with the complementary strand in the AES.
When the DNA was inserted via HIV-based vectors into stem cells from two strains of mice with sickle cell disorders, 99% of the red blood cells in the mice expressed the protective gene for up to 10 months, with no signs of sickling.
The amplified DNA was inserted into the pCR-Blunt vector (Invitrogen) creating the vector pCR 2.1-MSP142 3D7.2.
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In editing, DNA is inserted, deleted, or replaced in the genome using engineered or designer nucleases.
The next major event is integration, whereby the viral DNA is inserted into the host chromosome to create the proviral state.
Findings achieved with the new technique suggest that the letters of the four-letter code that constitutes the DNA are inserted in sequences more commonly than assumed, while frequency of deletions has been over-estimated.
The developmental pathway for complex double-stranded DNA viruses is generally conserved in the prokaryotic and eukaryotic groups and includes a genome packaging step where viral DNA is inserted into a pre-formed procapsid shell.
Hybrid Embryos: A reporter noted Willetts had missed a key vote on whether the United Kingdom should allow research into so-called hybrid embryos in which human DNA is inserted into animal eggs.
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