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The recombinant DNA was handled in accordance with the NIH guidelines.
DNA extraction from museum samples was carried out in a dedicated ancient DNA laboratory, physically separated from the laboratory where contemporary DNA was handled.
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In order to do so, we first analyse the literature dealing with the way DNA is handled by the various machineries in bacteria, explore the diversity of the corresponding processes and then try to see whether, despite this diversity, some common features emerge.
To avoid contamination, DNA of the positive controls and the patients was handled separately.
On the positive side, the Microsoft-Yahoo! combo could be a good force against Google's domination if the DNA mismatch can be handled.
The designed DNA duplication incision replacement process is handled by KF polymerase and endonuclease, then combining with gold nanoparticles as signal carrier for further signal amplification.
(This discussion assumes DNA, but RNA is handled identically, substituting U for T).
To verify the authenticity of amplificates from historical samples, all the mtDNA PCRs of museum samples were repeated by another person in a separate laboratory in a plant genetics department, where animal DNA had never been handled.
In yeast, large networks of genetic interactions have been shown to be substantially rewired by different types (Guenole et al. 2013) and levels of DNA damage (Srivas et al. 2013), suggesting that the interaction pattern itself is a sensitive measure of how DNA damage is being handled and by what subpathways.
A de novo human artificial chromosome (HAC) was constructed with naked human centromeric repetitive DNA (Harrington et al. 1997; Ikeno et al. 1998) and a HAC vector system developed in which one copy of a DNA fragment can be handled by Cre/lox insertion and transferred into a variety of vertebrate cell lines (Ikeno et al. 2009; Iida et al. 2010).
Extraction of ancient Huia DNA was performed in a dedicated ancient DNA laboratory physically separated from where contemporary DNA and PCR products were handled.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com