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The interaction of complex 1 with calf thymus DNA (CT DNA) was further investigated by absorption and fluorescence spectroscopic methods.
Purity of isolated genomic DNA was further assessed by electrophoresis in agarose gel (0.8%).
The pooled DNA was further purified using the Ampure Magnet Beads according to the manufacturer's protocol (Beckman Coulter, Brea, CA, USA).
The DNA was further cleaned using PowerClean Pro DNA Clean-Up Kit (MO Bio Laboratories, Cat. No. 12997-50) and used for the library preparation for subsequent sequencing.
After removal of the oil phase, the amplified DNA was further purified with SPRIselectTM beads in accordance with the ThunderBolts Cancer Panel Manual RainDanceTM Technologiess).
The metal ion selectivity of the catalytic DNA was further improved using a 'negative selection' strategy where catalytic DNA that are selective for competing metal ions are discarded in the in vitro selection processes.
The integrity and purity of extracted DNA was further assessed on the 1.0% agarose gel and measured using a Nanodrop spectrophotometer (Nanodrop 2000, Thermo Scientific, Japan).
The DNA was further stored at 4°C.
Precipitated DNA was further purified with PCR purification according to manufacturer's instructions (Qiagen).
Finally, the viral DNA was further purified using an UltraClean DNA Purification Kit (MBI).
The nonspecific nature of binding of MA to DNA was further demonstrated by PRE experiments.
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