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Further, the assembled M. mycoides genomic DNA was designed to include a gene encoding resistance to the antibiotic tetracycline, a gene not otherwise found in M. capricolum.
In order to further improve the sensitivity, the probe DNA was designed specially.
The target DNA was designed from influenza A virus sequence shown in GenBank database (http://www.ncbi.nlm.nih.gov/nuccore/401716582, accessed March 20 , 2014.
The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene.
Firstly, a double strand DNA was designed with 5′-SH modification of probe DNA and 5′-OH mofificatargetf target DNA.
In this study, a label-free fluorescence detection method for DNA was designed using functional magnetic nanoparticles (MNPs) as affinity probes.
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In the signal-on method the probe DNA is designed as stem-loop structure.
Four modified pairs of primers, designed to amplify the converted DNA, were designed using the MethPrimer software [37] (Table 2).
PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species.
In our system, the 5′ and 3′ ends of the template DNA were designed to hybridize with the middle region of the target DNA, leaving both ends of the target DNA as flexible overhangs.
The DNA-GA uses the DNA encoding method to represent the potential parameters and genetic operators inspired from the biological DNA are designed to find the global optimum.
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