The behavior of DNA under these conditions, in which the DNA is captured from the high-pressure side of the membrane, can be anticipated via finite element calculations.
In brief, methylated DNA was captured by methyl-CpG binding domain protein 2 (MBD2) coupled to magnetic beads to separate the modified from the non-modified DNA fractions.
Genomic DNA was captured in the region chr20.GRCh37 g.57200800_57627000 using a targeted SureSelectXT array (Agilent, 0.5-2.9Mb capture).
Normally, this approaches involves a "capture and release" system, in which the target DNA is captured by functionalised magnetic particles, separated from the sample matrix and then released in a suitable aqueous medium prior to detection with the polymer-probe duplex.
Exome library was captured from 3 µg of genomic DNA using Agilent SureSelect XT Human All Exon v.4 kit.
In the presence of polymerase (polδ), loaded PCNA is captured from DNA-bound RFC which subsequently dissociates, leaving behind the holoenzyme.
The new sequence from the tulip tree helps to understand what genes were present in the ancestral flowering plant mitochondrial genome, which ones were clustered together, which ones were captured from chloroplast DNA and when, which ones contained introns, and the degree to which each gene required editing of its RNA transcripts.
DNAP DNA complexes are captured from the bulk phase in the cis chamber, one complex at a time; tens to hundreds of individual complexes are examined, sequentially, in each experiment.
Protein-DNA complexes were captured by fixing heart homogenates from adult wild type or mIGF-1 Tg mouse at resting state in 1% (v/v) formaldehyde (Sigma) for 10 min at room temperature with gentle shaking.
