The concentration of fungal DNA was calculated by comparing a dilution series with defined amounts of DNA of lambda phage (methylated, from Escherichia coli host strain W3110).
To this end, the percent of precipitated DNA was calculated for each amplicon and then divided by the percent of precipitated DNA obtained with the amplicons from the internal positive control.
Based on the CV and spectroscopic results, the binding constant between NTZ and DNA was calculated to be 4.1 × 105 M−1 and 5.0 × 105 M−1, respectively.
The average ratio of mitochondrial to nuclear DNA was calculated from the results of triplicated reactions in each experiment, and the values were normalized to that of non-treated cells.
In pH 4.0 phosphate buffer solution (PBS), the binding ratio between STS and salmon sperm DNA was calculated to be 1 1 and the binding constant was 1.67 × 104 L/mol.
The binding constant (K) between LPT and DNA was calculated as 6.03 × 105 M−1, 4.20 × 105 M−1 and 3.50 × 104 M−1 for electrochemical, UV vis spectrophotometric and fluorescence spectroscopic techniques, respectively.
Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the value of (G + C /(A + T) of DNA was calculated as about 0.77 for various DNA samples.
The percentage of BamHI and EcoRI digestible PCR products among total DNA was calculated after quantifying DNA bands using Image J. pCEP4-Flex1 or pCEP4-Luc plasmids contain the Epstein-Barr virus (EBV) replication origins and propagate as episomes in mammalian cells10,67.
The amount of DNA was calculated according to the linear calibration curve of DNA (F = 2 × 10−4 C + 0.0437 R = 0.999 4).
The absorbance of the purified plasmid DNA was measured at 230, 260, 280, and 320 nm, and the mass concentration (C m, ng/μL) of DNA was calculated through the equation: C m = (A260 − A230) × 50, where A260 and A320 are the absorbance at 260 and 320 nm, and A320 was subtracted as background absorption.
The mass fraction of phosphorus (P %) in plasmid DNA was calculated to be 10.22 %, using the equation P % = 2×bp × 30.974/MDNA, Where bp is the number of base pairs in plasmid molecules, and MDNA is the molecular weight of the plasmids.
