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The consortium's senior scientists predicted in December 1998 that Dr. Venter's method of reassembling the sequenced fragments of genomic DNA was bound to fail.
Only a small fraction of cell-internalized SV40 that contained viral DNA was bound by the two importins.
However, probes pools were annealed to the biotinylated 3C samples and biotinylated DNA was bound on to streptavidin magnetic beads in 3D-DSL assay.
In preliminary experiments it was established that all of the junction DNA was bound by RuvC under these reaction conditions (data not shown).
After spinning, single stranded, bis-converted DNA was bound to the membrane.
After, the converted single strand DNA was bound to the membrane of the EpiTect spin columns.
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The DNA is bound by the clamp head and by the protrusion domain, allowing visualization of the upstream and downstream DNA duplexes in one of the elongation complexes.
This region, which is disordered in the X-ray models where an abasic site-containing DNA is bound to Fpg, interacts tightly with the 8-oxoG which appears to be confined within the enzyme.
In T7 RNA polymerase these changes involve refolding and reorientation of elements of the N-terminal domain, as well as changes in how the DNA is bound within the complex.
In very stable OC formed by the wild type WT RNAP with λPR (RPO) and by Δσ1.1 RNAP with λPR or T7A1, we conclude that downstream duplex DNA is bound to the jaw in an assembly with SI3, and bases − 4 to + 2 of the nontemplate strand discriminator region are stably bound in a positively charged track in the cleft.
After blocked with 6-mercapto-1-hexanol, the probe DNA is bound with the addition of target DNA to form the double-stranded structure on the electrode surface, which leads to a significantly decrease of peak current of electrochemical indicator [Fe CN 6]3−/4−.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com