The integrity of the DNA samples was ascertained by positive amplification of human DNA ß-globin control.
The quality of the DNA sample was ascertained by electrophoresis and determined to be of high quality (size >23 kb) with no visible degradation.
Lack of genomic and foreign DNA contamination was ascertained by using all RNA samples and water as a template in a PCR.
Lack of genomic and foreign DNA contamination was ascertained by using all RNA samples and water as a template in a PCR reaction.
Combinability of DNA partitions was ascertained using Wien's [ 101] method: existence of corresponding but incongruent clades with bootstrap support greater than 70% are seen as support for not concatenating data sets.
The relative of orientation of MA on the DNA in the complex was ascertained from residual dipolar coupling measurements, and this data, together with the chemical shift perturbation map, allowed us to generate a model of the complex.
DNA purity and concentration was ascertained by use of a Nanodrop™ 1000 spectrophotometer.
Zygosity was ascertained through DNA testing and parent ratings of twin resemblance [ 23].
The mechanism of peptide action on DNA synthesis and cell survival was ascertained.
