Sentence examples for dna verification from inspiring English sources

This breeding program provided the advantages of DNA verification of the paternity of the offspring, and diagnosed pregnancies by veterinary palpation, instead of just relying on non-return rates 60 90 days after breeding.

This program provides certain benefits, including DNA verification of the paternity of offspring and pregnancy diagnoses by veterinary palpation, instead of relying solely on non-return rates 60 90 days after breeding.

Forward and reverse primers were designed according to the SIGnAL T-DNA verification primer design tool (http://signal.salk.edu/tdnaprimers.2.html) for the SALK lines and with Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi) for the GABI line.

After DNA sequence verification, the cDNA were ligated to the pET-28b vector (Novagen), which was already ligated to the GFP cDNA.

Each plasmid will be grown in a small scale (~10 ml culture), purified and used for DNA sequence verification.

After the DNA sequencing verification, the insert was subcloned into pGL3-basic reporter vector within XhoI/ HindIII sites.

As a result, shRNA expression and red fluorescence reporter plasmids could be prepared without the need for colony selection and DNA sequence verification.

The primers used to isolate the BAC clones were used to amplify the same amplicon on 20 ng BAC DNA for verification.

After DNA sequence verification, deletion cassettes were amplified using primers P1 and P4 (Additional file 4: Table S2); purified with PCR Clean kit (Qiagen, USA) and concentrated by ethanol precipitation.

LysA, LysA2, LysgaY and λSa2 endolysin genes were sub-cloned into the pET21a E. coli expression vector (EMD Biosciences, San Diego, CA) and maintained in E. coli DH5α (Invitrogen, Carlsbad, CA) at 37°C in LB medium supplemented with 150 μg/ml ampicillin for plasmid purification, maintenance and DNA sequence verification.

To further facilitate testing of RNAi efficacy, we developed a negative selection marker (ccdB) method for construction of shRNA and red fluorescent reporter plasmids that allowed us to purify these plasmids directly from transformed bacteria without the need for colony selection and DNA sequencing verification.