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The plasmid was detected and reextracted from B. henselae EryR during three subcultures, but this plasmid was unstable in the new recipient since the quantity of plasmid DNA using the same amount of bacterial biomass decreased after each subculture (Figure 6).
Experimental qPCR values were normalized against values obtained for 25 ng of input DNA using the same primer set.
As an internal control, we supplemented the libraries at a 1% ratio with library molecules made from Drosophila melanogaster genomic DNA using the same vector.
The first strand cDNA was then synthesized to double-stranded DNA using the same primer and Klenow DNA polymerase (Takara, Dalian, China).
The α chain probe was designed from the exon 2 of a tammar wallaby DAA gene ([GenBank: CU464025], position 141572-141757) amplifiedfrom from tammar wallaby genomic DNA using the same PCR reagents and conditions as described above.
To obtain Pacific Bioscience (PacBio; Menlo Park, CA) continuous long reads (CLRs), we extracted genomic DNA using the same phenol chloroform extraction as used to extract gDNA for the Illumina sequencing from a brother/sister pair of adult beetles that had been inbred for six generations.
Similar(52)
Association results with the pooled DNA method and following re-genotyping of individual DNAs using the same set of 1,000 screened individuals, 23 markers showed significant differences by Fisher's exact test (Table 1).
Quantitative real-time PCR was performed to detect either HPV16 E6 DNA or E6 cDNA using the same primer/probe set for both.
We re-amplified aliquots of the DNA by using the same amplification kit and purified the DNA with a standard ethanol precipitation protocol (Note: previous work in our lab had shown that genotype data of pre-amplified and re-amplified genomic DNA from Nasonia embryos are fully consistent).
U2M.L (universally unmethylated DNA) was generated as a negative control by amplifying the same DNA used to generate the positive control DNA using the GenomiPhi kit (GE Healthcare) as described by the manufacturer.
Regarding rat (Rattus norvegicus) and rabbit (Oryctolagus cuniculus) species, we failed to amplify DNA fragments using the same pairs of oligonucleotide primers that amplified the pig sequence.
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CEO of Professional Science Editing for Scientists @ prosciediting.com