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To investigate DNA uptake, 20 μg of DNA was labeled with Cy5.5-NHS as suggested by manufacturer's protocol.
Hence, we find a clear association between the acquisition of virulence factors and DNA uptake as well as genome size, suggesting that the pathogenic potential of E. coli is associated with DNA uptake.
The conventional approaches of plant genetic transformation include Agrobacterium tumefaciens, particle bombardment, DNA uptake into protoplast.
Protocols for protoplast formation and regeneration were established; however, preparations were recalcitrant to DNA uptake.
When DNA uptake changes the cell's genotype, the cell is said to be transformed.
DNA uptake of hepatocytes decreased by immobilized HEGFP whereas it increased by soluble EGF.
These bacteria express competence for DNA uptake and the uptake process is called natural transformation.
Here we present experimental models that examine DNA uptake in bacteria, their advantages and limitations.
In E. coli, there are homologues of the competence genes that other species use for DNA uptake and processing.
In vitro studies indicate that enhancing cell proliferation with growth factor exposure enhances plasmid DNA uptake and expression.
We found that electroporation promotes a highly efficient DNA uptake by human primary cultures of myogenic cells.
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