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The gel slice was incubated in TE buffer plus 100 mM NaCl overnight to allow the DNA to diffuse into the solution.
S/MARs were isolated by either 2 m NaCl or 25 m m LIS to remove histones thereby enabling unconstrained DNA to diffuse away from the nuclear scaffold/matrix forming a peripheral halo (Fig. 1A).
In the second, the agarose gel slice was incubated for at least 24 h in 3× (w/v) gel extraction buffer (300 mM NaOAc and 1 mM EDTA) while being shaken at 37 °C to allow the DNA to diffuse out of the gel.
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The flat, hydrophilic surface facilitates ink and small DNA fragments to diffuse on the substrate, leading to an enlarged gap and a clear view field.
DNA fragments were extracted from the denaturing gel by immerging the gel bands in 50 μl of sterile water, and allowed to stand overnight at +4°C in order to allow the DNA fragments to diffuse.
Did you use humor as a dad to diffuse situations?
The complementary DNA was left unperturbed to diffuse and hybridize to the chimera DNA for at least 2 min. The hybridized chimera channel was then depolarized following the same protocol of ssChi and the currents were measured repeatedly for 3 5 times.
The DNA template is allowed to diffuse into the ZMV in the presence of primer and nucleotides with fluorescent labels attached to the phosphate chain.
These observations confirm that the ability of AAG to diffuse on DNA does not require the amino terminus and that diffusion of the truncated protein is not blocked by these DNA structures.
Previous work showed that AAG uses electrostatic interactions to diffuse along DNA and search for sites of damage.
The p53 protein, a transcription factor of key importance in tumorigenesis, is suggested to diffuse one-dimensionally along DNA via its C-terminal domain, a process that is proposed to regulate gene activation both positively and negatively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com