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CRISPR-Cas9 is a hybrid of protein and RNA – the cousin to DNA – that functions as an efficient search-and-snip system in bacteria.
Another requirement for carrying out RCA is hybridization between the closed circular DNA and primer DNA that functions as an initiation site of DNA polymerase.
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SPG7 is associated with multiple mitochondrial DNA deletions, suggesting that functions of other mitochondrial proteins involved in either mitochondrial DNA replication itself or pathways of mitochondrial quality control are altered (Wedding et al. 2014).
Proteins in the RecA/RadA/Rad51 family form helical filaments on DNA that function in homologous recombination.
The molecular gene concept stems from the idea that genes are units in DNA that function to determine linear sequences in molecules synthesized via DNA expression.
In addition, OMVs are known to possess LPS and bacterial DNA that function as ligands for host PRRs [44], [45].
A start-up company has even been set up to create what its founders call "genetic applets," pieces of DNA that handle functions analogous to those handled by pieces of computer code.
Interestingly, VACV also encodes a uracil DNA glycosylase that functions as a processivity factor and interacts with the VACV A20R protein during genome replication [11], [12].
POLE and POLD1 encode DNA polymerases that function in DNA replication.
The main DNA ligases that function in mammalian cells include DNA ligase I (DNA replication and repair) [97,98], DNA ligase III (base excision repair) [62] and DNA ligase IV (non-homologous end joining) [99 101].
The Voigt lab made a point of ensuring that the designs would work as expected, where the software could reliably design DNA that would function in the cell as simulations predicted.
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