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Proteins in the RecA/RadA/Rad51 family form helical filaments on DNA that function in homologous recombination.
The molecular gene concept stems from the idea that genes are units in DNA that function to determine linear sequences in molecules synthesized via DNA expression.
In addition, OMVs are known to possess LPS and bacterial DNA that function as ligands for host PRRs [44], [45].
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Another requirement for carrying out RCA is hybridization between the closed circular DNA and primer DNA that functions as an initiation site of DNA polymerase.
POLE and POLD1 encode DNA polymerases that function in DNA replication.
The main DNA ligases that function in mammalian cells include DNA ligase I (DNA replication and repair) [97,98], DNA ligase III (base excision repair) [62] and DNA ligase IV (non-homologous end joining) [99 101].
The Voigt lab made a point of ensuring that the designs would work as expected, where the software could reliably design DNA that would function in the cell as simulations predicted.
DSBR has recently been proposed to result in intron gain if NHEJ inserts filler DNA that may function as a spliceable intron [ 4, 58].
Phage display results in linkage of an affinity-selectable function (the displayed peptide) to the DNA encoding that function, allowing selection of individual binding clones by interative cycles of in vitro panning and in vivo amplification.
Synthetic biologists have created DNA chains that function, but are made of different sugars and acids than the stuff in our cells.
It is therefore possible that TAR may occasionally insert DNA segments that function as introns.
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