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The LAMP mix without DNA templates (Table 3) was first prepared on ice.
The concentrations of standard DNA templates for conventional PCR and real-time PCR were the same.
The sequencing primer was then annealed to the immobilized amplified DNA templates.
In addition, this method can facilitate simultaneous identification of multiple DNA templates.
The detection range of the method was assessed on purified DNA templates from different animal origins.
Background cross-amplification with DNA templates derived from 14 other species was negligible.
The enzyme also ligated double-stranded (ds) DNA templates, a novel activity not previously reported.
Under optimal conditions, 0.001 ng DNA templates were enough to generate miniSTRs.
DNA templates with ASOs-complementary sequences were amplified by rolling circle amplification (RCA).
Employing engineered DNA templates to express antiviral microRNA (miRNA) sequences has considerable therapeutic potential.
DNA templates from the kDNA-positive/T.
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