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Figure 8 SEM image of DNA template nanowires.
Genomic DNA was used as DNA template in amplification process.
This approach does not require any natural DNA template.
Too much IAC DNA template would out-compete the target DNA template, thus giving a false negative result.
CIS display noncovalently links RepA (DNA-binding protein) fusion protein and its coding DNA template through the interaction between RepA and the CIS element of the DNA template.
Instead of 2.5 μl of 5 ng/μl DNA template, 2.5 μl of 60-fold diluted DNA template was used for the qPCR.
If, however, the DSB occurs before replication, no such DNA template is available.
Recent advances include emulsion-PCR based DNA template preparation, the design and synthesis of novel reporter nucleotides and new surface attachment chemistries for DNA template.
No product was detected when the DNA template was replaced with ddH2O.
The DNA template used for RNA synthesis was y38f2ar.9 (YAC Y38F2AR: nt 56335 56636).
−CT, negative control without addition of the genomic DNA template; +CT, with added plasmid DNA.
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